Inno-lipa hbv genotyping manual

 · Hepatitis B virus (HBV) is a major cause of liver cirrhosis and hepatocellular carcinoma (HCC). Eight different HBV genotypes have been identified with distinct geographical distributions. Different genotyping methods exist including sequencing, INNO-LiPA, restriction fragment polymorphism (RFLP), multiplex PCR, serotyping, oligonucleotide. The INNO-LiPA HPV Genotyping Extra is based on the principle of reverse hybridization. Part of the L1 region of the human papillomavirus (HPV) genome is amplified using SPF10 primers, and the resulting biotinylated amplicons are then denatured and hybridized with specific oligonucleotide probes. INNO-LiPA ® HBV Genotyping When genotyping, be confident INNO-LiPA HBV Genotyping is a line probe assay designed to identify hepatitis B virus genotypes A to H by the detection of type-specific sequences in the HBV polymerase gene domain B to C.

INNO-LiPA ® HBV Genotyping Product number - Line probe assay designed to identify hepatitis B virus genotypes A to H by the detection of type-specific sequences in the HBV polymerase gene domain B to C. The INNO-LiPA HBV Genotyping assay is a line probe assay designed to identify hepatitis B virus (HBV) genotypes A to H by detection of type-specific sequences in the HBV polymerase gene domain B to C. HBV Genotype Assay (LiPA) is not intended to be used as a screening test for HBV or as a diagnostic test to confirm the presence of HBV. In this study, the INNO-LiPA assay identified 28 mixed infections in a population of HBsAg- and anti-HBcAg-hepatitis B virus (HBV)-DNA-positive French blood donors. Nine of the 28 mixed infections included genotype H as part of a double or triple infection. Sequencing after molecular cloning did not confirm the presence of genotype H in.

2 Agu Different genotyping methods exist including sequencing, INNO-LiPA, restriction fragment polymorphism (RFLP), multiplex PCR, serotyping. YMDD variants were analyzed using the HBV drug resistance line probe assay (Inno-Lipa HBV-DR). HBV genotypes were detected with polymerase chain reaction. INNO-LiPA HBV Genotyping is a line probe assay designed to identify hepatitis B virus genotypes A to H by the detection of type-specific sequences in the.