Abi identifiler plus manual

10 lLofAmpF‘STR Identifiler Plus Master Mix, 5 lLof AmpF‘STR Identifiler Plus Primer Set, and a maximum vol-ume of 10 lL of target DNA. Samples were amplified in Micro-Amp reaction tubes or MicroAmp Optical well reaction plate (Applied Biosystems) in the GeneAmp PCR system with a gold-plated silver or silver block (Applied. The Anne Arundel County Crime Lab is upgrading from an Applied Biosystems ® (AB) genetic analyzer to an AB genetic analyzer. The internal validation of this AB included a comparison of the PowerPlex ® 16 HS and Identifiler ® Plus amplification kits using the manufacturers recommended protocols to determine if one. Identifiler, Identifiler Plus* (aSTRs) amelogenin 15 autosomal STRs Life Technologies (Applied Biosystems) Covers the 13 core CODIS loci plus 2 extra PowerPlex 16 D3S PowerPlex 16 HS* 15 aSTRs amelogenin Promega Corporation Covers the 13 Analyst Manual Review Software (e.g., GeneMapperID) Expert System.

Identifiler Electrophoresis and Analysis on the ABI xl Genetic Analyzer Status:Published Document ID: DATE EFFECTIVE 01/10/ APPROVED BY Nuclear DNA Technical Leader PAGE 2 OF 28 Controlled versions of Department of Forensic Biology Manuals only exist in the Forensic Biology Qualtrax software. All printed versions are non-controlled. Identifiler™ Plus kits. These improvements enhance the assay signal-to-noise ratio and simplify the interpretation of results. The primers used in the kits are: • For all loci except AMEL—The same primer sequences as the NGM SElect™ kit and the Identifiler™ Plus kit including SNP-specific primers for the vWA. PowerPlex™ 16 HS proved more sensitive than Identifiler™ Plus. Identifiler™ Plus exhibited better heterozygote peak balance and mixture elucidation. Due to 5 dye chemistry of Identifiler™ Plus the loci are spread more evenly eliminating the visual “merging” of loci as can happen in PowerPlex™ 16 HS if you aren’t used to it.

AmpFlSTR Identifiler PCR Amplification Kit. User's Manual (Applied Biosystems, ). Tvedebrink, T., Eriksen, P. S., Mogensen, H. S. . GeneMapper™ ID version Upgrade Kit for ABI PRISM™ Genetic Analyzer AmpFLSTR™ SEfiler PCR Amplification Kit User's Manual and CD. Applied Biosystems® fluorescent multi-color dye technology allows the analysis of multiple loci, including loci that have alleles with.